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KMID : 0384119930130020247
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 2 p.247 ~ p.258
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A Study on the Serum and Cell Lactate Dehydrogenase Isoenzyme in Hematologic Malignancies
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Abstract
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Lactate dehydrogenase(LD;EC 1.1.1.27) is a tetramer composed of two types of subunit, H and M, and has five distinct isoenzymes according to the composition of their subunits. The proportion of these isoenzyme differs in various organs.
Alterations
in
the proportions of the LD isoenzymes have been observed during development, under changing biological conditions, and in response to pathological processes. Elevation of serum LD have been described especially in leukemia and lymphoma, and have
been
known useful indicator of tumor burden and prognosis. In this study, the measurements of serum LD activity and LD isoenzyme pattern of leukemic cells and sera have been performed to find whether intracellular LD isoenzyme pattern could
discriminate
between acute myeloid leukemia(AML) and acute lymphoblastic leukemia(ALL). And also we tried to know whether LD isoenzyme reflected to distinguish leukemic cells and normal cells. LD isoenzyme pattern in all sera of AML and ALL revealed
isomorphic
pattern or only LD3 elevation or LD3 woith LD4 elevation. Also LD isoenzyme pattern of leukemic cells from both AML and ALL were identical, demonstrating picture with predominance of LD3. LD isoenzyme pattern in sera and leukemic cells of acute
leukemia
were quite different. We concluded that serum LD activity and its isoenzyme were significant biochemical marker in diagnosis of acute leukemia and significant contribution to elevation of serum LD might come from nonleukemic sources. Also
distribution
of LD isoenzyme were quite different in leukemic blasts, normal granulocyte and lymphocyte. LD isoenzyme pattern in cell could not discriminate between AML and ALL, and between T-ALL and non-T ALL.
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KEYWORD
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